The objective was to develop a simple, economical, accurate and precise stability indicating RP-HPLC for simultaneous estimation of levofloxacin and ambroxol in combined tablet dosage form. Stability indicating RP-HPLC method was developed and validated for simultaneous estimation of levofloxacin and ambroxol using ACE C18 column (5 µ,150 × 4.6mm) and methanol: buffer KH2PO4 (pH 5 ) in a volume 35:65 v/v as mobile phase with 221 nm. Stability indicating RP-HPLC method was developed and validated. The method successfully separated levofloxacin and ambroxol from its degradation product formed under stressed conditions. Levofloxacin and ambroxol degraded significantly under acidic, alkaline and oxidative conditions. The method was found to be linear in range at 20-100 μg/ml of LVF and 3-15 μg/ml of AMB and the % recovery was found in the range of 98.43 - 98.72% and 99.18 - 99.99% and % assay was found to be 99.46 and 100.54 for LVF and AMB, respectively. The proposed SIAM method was found to be simple, accurate, economical, robust and reproducible.
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